Table 1. Forward and reverse primers sequence used for RT-qPCR.

Gene	Primer sequences	Amplicon size
Human β- Actin	F: 5′ ggc atc ctc acc ctg aag ta 3′	82 bp
	R: 5′ agg tgt ggt gcc aga ttt tc 3′
Human Runx-2	F: 5′ gta gat gga cct cgg gaa cc 3′	78 bp
	R: 5′ gag gcg gtc aga gaa caa ac 3′
Human ALP	F: 5′ gga act cct gac cct tga cc 3′	86 bp
	R: 5′ tcc tgt tca gct cgt act gc 3′
Human Col1a1	F: 5′ gag tgc tgt ccc gtc tgc 3′	52 bp
	R: 5′ ttt ctt ggt cgg tgg gtg 3′
Human Type X Collagen	F: 5′ ccc act acc caa cac caa ga 3′	95 bp
	R: 5′ gtg gac cag gag tac ctt gc 3′
Human Osteonectin	F: 5′ gag gaa acc gaa gag gag g 3′	95 bp
	R: 5′ ggg gtg ttg ttc tca tcc ag 3′
Human SMAD3	F: 5′ tga atc cct acc act acc aga g 3′	117 bp
	R: 5′ gga tgg aat ggc tgt agt cg 3′
Human SMAD 2	F: 5′ gat cct aac aga act tcc gcc 3′	146 bp
	R: 5′ cac ttg ttt ctc cat ctt cac tg 3′
Human Sox9	F: 5′ ccc ttc aac ctc cca cac ta 3′	74 bp
	R: 5′ tgg tgg tcg gtg tag tcg ta 3′
Human Col2a1	F: 5′ cct ggt ccc cct ggt ctt gg 3′	58 bp
	R: 5′ cat caa atc ctc cag cca tc 3′
Human Nucleostemin	F: 5′ ggg aag ata acc aag cgt gtg 3′	98 bp
	R: 5′ cct cca aga agt ttc caa agg 3′
Human CD63	F: 5′ gcc ctt gga att gct ttt gtc g 3′	87 bp
	R: 5′ cat cac ctc gta gcc act tct g 3′
Human ALCAM	F: 5′ acg atg agg cag acg aga taa gt 3′	96 bp
	R: 5′ cag caa gga gga gac caa caa c 3′