Figure 5. Effect of blocking SAV-QD605s with biotin.

Box-and-whiskers plot of the diffusion coefficients for EGFR^BLAP targeted with SAV-QD605, with (dark gray) and without blocking (light gray) with excess biotin ½–1 min post SAV-QD605 labeling, respectively. The mean diffusion coefficient increased as indicated on the figure by ∼120%. The reduced mean diffusion coefficient when not blocking with biotin indicates that SAV-QD605 induced cross-binding of EGFR^BLAP.