Figure 4.

Spatial expression analysis of the NIN gene. GUS staining images of Agrobacterium rhizogenes-mediated transformed roots with ProNIN::GUS::TerNIN at 7 DAI (with the M. loti MAFF303099 wild type) on the Miyakojima MG-20 wild type (A, C, E, and G) and daphne (B, D, F, and H). Blue staining was observed with transformed (GFP-positive) hairy roots in susceptible zones (A–F), including root hair cells (G and H) and an immature nodule in the wild type (I). Bars = 0.5 mm in A to D and 0.1 mm in E to I.