Figure 5.

Ectopic expression of the NIN gene strongly suppresses excessive IT formation in daphne. Red fluorescence images (A–D), GFP fluorescence images (E–H), and transmitted light images (I–L) of A. rhizogenes-mediated transformed daphne roots are shown at 21 DAI. Roots were transformed with negative control vector ProLjUBQ::GUS (A, E, and I), ProLjUBQ::NIN (B, C, F, G, J, and K), or ProLjUBQ::NIN::SRDX (D, H, and L). ITs were observed by inoculating M. loti MAFF303099 constitutively expressing DsRED (A–D). GFP fluorescence showed transformed roots (E–H). Yellow dashed lines indicate the border between transformed and nontransformed roots. Bars = 2 cm.