Figure 9.

(A) Ultraviolet absorption spectra of amphotericin B in three different media. The four peaks observed in the monomeric amphotericin B are labelled. The concentration of amphotericin used was 50 μg/mL. The Y-axis for the amphotericin B in gelatin is represented separately. (B) The relative aggregation state of amphotericin B as measured by the intensity ratio of peak I and peak IV of the absorption spectra in three different media. (C) Circular dichroism spectrum of amphotericin B in three different media. Note the bisignate nature of amphotericin B in phosphate buffered saline indicating a complex structure. (D) Hemolytic activity of amphotericin B and natamycin in the presence/absence of gelatin. For all these studies, the antifungal:gelatin ratio was 0.25 wt%. (E) Ultraviolet absorption spectra of amphotericin B released from the gelatin matrix. For a comparison, the absorption spectra of free amphotericin B (10 μg/mL) in buffer is also shown.

Note: ***P<0.001.

Abbreviations: AmpB, amphotericin B; DMSO, dimethyl sulfate; PBS, phosphate buffered saline; AU, arbitrary unit.