Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control

. 2014 May 27;9:2635–2653. doi: 10.2147/IJN.S59834

Table of contents

Serial number	Contents	Page number
1.	Supplementary figure 1 HPTLC fingerprint of PZRE. (A) PZRE ethyl acetate extract, and (B) standard mixture of plumbagin (P), diosgenin (D), quercetin (Q) and catechin (C).	2650
2.	Supplementary figure 2 Bioreduction of AuNPs from PZRE at varying reaction temperatures and concentrations of the salts as a function of time. (A) Time course of AuNPs synthesis against reaction temperature, and (B) time course of AuNPs formation at different HAuCl₄ concentration.	2650
3.	Supplementary figure 3 Determination of purity, size and nature of the AuNPs synthesized by PZRE at 37°C. (A) Representative spot energy-dispersive spectrum, (B) histogram of size distribution, (C) zeta potential analysis, and (D) representative X-ray diffraction profile of thin film.	2651
4.	Supplementary figure 4 Determination of purity, size and nature of the AgNPs synthesized using 1% trisodium citrate. (A) Representative X-ray diffraction profile of thin film, (B) representative spot energy-dispersive spectrum, (C) transmission electron micrograph, and (D) histogram of size distribution.	2651
5.	Supplementary figure 5 Determination of purity, size and nature of the AuNPs synthesized using 1% trisodium citrate. (A) Representative X-ray diffraction profile of thin film, (B) representative spot energy-dispersive spectrum, (C) transmission electron micrograph, and (D) histogram of size distribution.	2652
6.	Supplementary figure 6 Determination of purity, size and nature of the AgAuNPs synthesized using 1% trisodium citrate. (A) Representative X-ray diffraction profile of thin film, (B) representative spot energy-dispersive spectrum, (C) transmission electron micrograph, and (D) histogram of size distribution.	2652
7.	Supplementary figure 7 AFM micrographs for biofilm disruption. (A) A. baumannii biofilm disruption using AgNPs using AFM, and (B) amplitude mode of AFM image.	2653
8.	Materials and methods for cytotoxicity assay	2653
9.	Supplementary figure 8 Cytotoxicity of the nanoparticles synthesized from PZRE and 1% trisodim citrate determined using MCF7 cells showing no inhibition upto the concentration of 0.5 mg/well.	2653

Abbreviations: HPTLC, high-performance thin-layer chromatography; PZRE, Plumbago zeylanica root extract; AgNPs, silver nanoparticles; AuNPs, gold NPs; HAuCl₄, chloroauric acid; AgAuNPs, bimetallic NPs; AFM, atomic force microscopy.