Table 2.
Effect of alkalization temperature
| T (°C) | Fe concentration (mM)
|
Encapsulation efficiency (%) | Final lipid content (mM) | Drug loading (mol Fe/mol lipid) | |||
|---|---|---|---|---|---|---|---|
| Before alkalization
|
After alkalization
|
||||||
| Feb | Fe(t) | Fe(f) | Fe(c) | ||||
| 30 | 35.2±0.8 | 32.0±1.5 | 17.9±1.0 | 14.2±0.5 | 9.4±0.3 | 19.5±0.3 | 0.73±0.03 |
| 40 | 34.5±0.5 | 31.3±0.9 | 11.5±1.1 | 19.8±0.4 | 13.2±0.3 | 19.1±0.3 | 1.03±0.03 |
| 50 | 35.0±0.7 | 30.8±1.8 | 4.3±2.5 | 26.5±1.2 | 17.7±0.8 | 19.1±0.4 | 1.39±0.04 |
| 60 | 34.3±0.6 | 29.7±0.4 | 3.1±0.5 | 26.6±0.6 | 17.7±0.4 | 18.2±0.3 | 1.46±0.04 |
Note: n=3.
Abbreviations: Feb, the amount of iron ions in liposomes before alkalization; Fe(t), the amount of total iron in the liposomes after alkalization; Fe(f), the amount of free iron ions in the liposomes after alkalization; Fe(c), the amount of iron-oxide cores in magnetoliposomes; T, temperature.