Table 3.
Effect of alkalization duration
| T (minutes) | Fe concentration (mM)
|
Encapsulation efficiency (%) | Final lipid content (mM) | Drug loading (mol Fe/mol lipid) | |||
|---|---|---|---|---|---|---|---|
| Before alkalization
|
After alkalization
|
||||||
| Feb | Fe(t) | Fe(f) | Fe(c) | ||||
| 15 | 34.4±0.4 | 30.5±0.8 | 11.1±1.1 | 19.4±0.3 | 12.9±0.2 | 18.4±0.5 | 1.05±0.04 |
| 30 | 34.3±0.6 | 29.7±0.4 | 3.1±0.5 | 26.6±0.6 | 17.7±0.4 | 18.2±0.3 | 1.46±0.04 |
| 60 | 34.6±0.6 | 28.5±0.5 | 0.8±0.2 | 27.7±0.4 | 18.5±0.3 | 17.5±0.6 | 1.58±0.04 |
| 120 | 34.2±0.4 | 26.0±0.5 | 1.0±0.3 | 25.1±0.4 | 16.7±0.3 | 17.1±0.2 | 1.46±0.04 |
Note: n=3.
Abbreviations: Feb, the amount of iron ions in liposomes before alkalization; Fe(t), the amount of total iron in the liposomes after alkalization; Fe(f), the amount of free iron ions in the liposomes after alkalization; Fe(c), the amount of iron-oxide cores in magnetoliposomes; T, time.