Table 6.
Results of orthogonal experiments
| Experiment number | A: initial Fe concentration (mM) | B: alkalization pH | C: alkalization temperature (°C) | D: alkalization duration (minutes) | E: random* | Drug loading (mol Fe/mol lipid) |
|---|---|---|---|---|---|---|
| 1 | 1 | 1 | 1 | 1 | 1 | 0.24 |
| 2 | 1 | 2 | 2 | 2 | 2 | 0.95 |
| 3 | 1 | 3 | 3 | 3 | 3 | 0.69 |
| 4 | 1 | 4 | 4 | 4 | 4 | 0.12 |
| 5 | 2 | 1 | 2 | 3 | 4 | 1.02 |
| 6 | 2 | 2 | 1 | 4 | 3 | 0.94 |
| 7 | 2 | 3 | 4 | 1 | 2 | 0.89 |
| 8 | 2 | 4 | 3 | 2 | 1 | 0.51 |
| 9 | 3 | 1 | 3 | 4 | 2 | 0.95 |
| 10 | 3 | 2 | 4 | 3 | 1 | 1.58 |
| 11 | 3 | 3 | 1 | 2 | 4 | 0.43 |
| 12 | 3 | 4 | 2 | 1 | 3 | 0.58 |
| 13 | 4 | 1 | 4 | 2 | 3 | 0.57 |
| 14 | 4 | 2 | 3 | 1 | 4 | 0.77 |
| 15 | 4 | 3 | 2 | 4 | 1 | 0.48 |
| 16 | 4 | 4 | 1 | 3 | 2 | 0.23 |
| k1 | 0.500 | 0.695 | 0.460 | 0.620 | 0.703 | |
| k2 | 0.840 | 1.060 | 0.757 | 0.615 | 0.755 | |
| k3 | 0.885 | 0.623 | 0.730 | 0.880 | 0.695 | |
| k4 | 0.512 | 0.360 | 0.790 | 0.622 | 0.585 | |
| R | 0.385 | 0.700 | 0.330 | 0.265 | 0.170 |
Note:
*
Random is the control factor of orthogonal experiment.
Abbreviations: k, the average drug-loading value of magnetoliposomes at different levels of every factor; R, the range of k at different levels of every factor.