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. 2014 May 24;8:583–600. doi: 10.2147/DDDT.S60283

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© 2014 Wu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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RBC1023 inhibits STAU-induced caspase-3 cleavage and activation in NIH3T3 cells.

Notes: NIH3T3 cells were treated with DMSO, 20 μM of RBC1023, 1 μM of STAU, or 20 μM of RBC1023 plus 1 μM of STAU for 6 hours. Caspase activity was measured by Apo-ONE^® Homogeneous Caspase-3/7 Assay (Promega Corporation, Fitchburg, WI, USA) (A), and processing of caspase-3 was detected by Western blotting with anti-caspase-3 antibody (B). Positions of the intact and cleaved forms of capase-3 are indicated by arrows. α-tubulin was used as a control for protein loading. One-way analysis of variance indicates: P>0.05 (DMSO versus [vs] RBC1023), P>0.05 (DMSO vs RBC1023 + STAU), and P<0.01 (STAU vs RBC1023 + STAU).

Abbreviations: DMSO, dimethyl sulfoxide; STAU, staurosporine.