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. 2014 Jun 3;9(6):e98686. doi: 10.1371/journal.pone.0098686

Figure 9. Neutral comet assay compatible with a DNA double-strand break repair disorder.

Figure 9

(a). Tail moment of WT, A-T and patient cells at 0, 30 minutes and 5 hours post-irradiation. All cells show damage at 30 minutes post-irradiation. WT returns to near baseline levels after 5 hours, while A-T and patient cells show long comet tails after 5 hours, indicating significant levels of unrepaired DNA. (b). Ratio of unrepaired DNA at 5/0 hours. A-T as well as Patient 2 and 3 cells show statistically significantly lower levels of repair at 5 hours post-irradiation compared to wild type cells (* p<0.05). Experiments were performed in triplicate with the average of three experiments shown. WT  =  wild type; A-T  =  ataxia-telangiectasia control.