(Top) Schematic of the transcription of the four osmolyte transporters in L. monocytogenes after cold shock. B, betaine; C, carnitine. (Bottom) RNA was isolated from mid-exponential-phase cells prior to cold shock (lanes labeled 37) and from cells exposed to 10°C for 30 min (lanes labeled 10). The results after 30 cycles are shown for gbu (Gbu), betL (BetL), opuC (OpuC), and opuB (OpuB).