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. 2014 Jun 3;9(6):e98737. doi: 10.1371/journal.pone.0098737

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© 2014 Chujo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Expression analysis of momilactone biosynthetic genes. qRT-PCR analysis was performed using total RNA isolated from uninfected rice leaves. Values indicate relative mRNA levels normalized to the expression of the UBQ gene (n = 3); bars indicate the standard error of the mean. Three independent experiments were performed, and a representative result is shown. B, Accumulation of momilactones in uninfected W53SD-OX rice plants. Momilactone levels in the rice leaves were determined by LC-MS/MS. The results are the average of at least 3 independent experiments. Bars indicate the standard error of the mean. NT, non-transformed control; OX, OsWRKY53-overexpressing rice plants; SD-OX; OsWRKY53SD-overexpressing rice plants. N.D.: not detected.