FIG. 2.

Images of the T. atroviride pki1::gfp mutant colonizing a cucumber root (A and B) and soil particles (C) in sterilized soil inoculated with P. ultimum after 3 days of incubation. The initial concentrations of P. ultimum and T. atroviride added to the soil were 3.1 × 10³ propagules g⁻¹ and 3 × 10³ spores g⁻¹, respectively. Cucumber seeds were also planted at the time of inoculation. The images in panels A and B were taken with a CSLM with a ×20 objective. The arrows indicate green fluorescent T. atroviride hyphae alongside P. ultimum hyphae (yellow or reddish brown) on the outer surface of a cucumber root (reddish brown). The image in panel C was taken with a fluorescence steromicroscope by using a ×6.9 objective and shows green fluorescent T. atroviride hyphae (arrow) adhering to soil particles (brownish or yellow).