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. Author manuscript; available in PMC: 2015 Mar 21.
Published in final edited form as: Chembiochem. 2014 Mar 21;15(5):701–712. doi: 10.1002/cbic.201300625

Figure 3.

Figure 3

Expression and activity of ALDH1A1 and ALDH3A1 in A549, SF767, and CCD-13Lu cells. Lysates from various cancer cell lines (A549, SF767and CCD-13Lu) were examined for (A) ALDH3A1 expression and (B) ALDH1A1 expression. (C) Western blot of purified human ALDH1 and ALDH2 isoenzymes using the Abcam (ab-23375) ALDH1A1 antibody. Each lane was loaded with between 50 and 70 ng of the purified recombinant human ALDH1A1, ALDH1A2, ALDH1A3, ALDH1B1 and ALDH2 isoenzymes, respectively. (D) Recombinant ALDH3A1, A549, SF767 and CCD-13Lu cell lysate activity were tested in the presence of 1.5 mM NADP+ and 1 mM Benzaldehyde and in the presence and absence of 50 μM CB29 for 3 minutes. Unlike A549 and SF767 cells, CCD-13Lu cell lysate did not have dehydrogenase activity. The p values were calculated using the Student’s t test comparing activity in the absence and presence of inhibitor (CB29) (*, p < 0.001, n=3).