Table 2.

Classification of coexpression patterns of VGCC α1 subunits and xVGlut1

	Forebrain	Midbrain	Hindbrain	Spinal Cord	Retina	Cranial Nerves
% Cav1.2 cx	+	++	+	+	-	-
% vGlut cx	++	++	+	++	-	-
% Cav1.3 cx	+	-	+++	+++	+	+++
% vGlut cx	++	-	++	+++	+	+++
% Cav1.4 cx	N/A	N/A	N/A	N/A	+	N/A
% vGlut cx	N/A	N/A	N/A	N/A	+	N/A
% Cav2.1 cx	+	++	+	+	+	+++
% vGlut cx	++	+++	+	++	++	+++
% Cav2.2 cx	+	+	++	+++	-	+++
% vGlut cx	+	++	++	+++	-	+++
% Cav2.3 cx	N/A	+	+	-	N/A	+
% vGlut cx	N/A	+	+	-	N/A	+
% Cav3.1 cx	-	+	+++	+++	+	+++
% vGlut cx	-	+++	+++	+++	+	+++
% Cav3.2 cx	-	+	+	++	+	++
% vGlut cx	-	+	+	++	+	++

For each coexpression (cx) experiment, the relative percentage of cells expressing both the VGCC subunit and xVGlut1 (yellow FISH signal in Figs. 1–2) out of the total number of cells expressing the VGCC subunit (green and yellow FISH signal in Figs. 1–2) was determined. Additionally, the relative percentage of cells expressing both the VGCC subunit and xVGlut1 (yellow FISH signal) out of the total number of cells expressing xVGlut1 (red and yellow FISH signal) was determined. A single plus sign (+) indicates that <25% of the cells expressing the marker displayed coexpression. Two plus signs (++) indicates that 25–75% of the cells expressing the marker displayed coexpression, and three plus signs (+++) indicates >75% coexpression. Full coexpression between the two markers (>90%) is indicated in bold. Experiments without coexpression are marked with a minus sign (−).