Table 3.

Classification of coexpression patterns of VGCC α1 subunits and xVIAAT

	Forebrain	Midbrain	Hindbrain	Spinal Cord	Retina	Cranial Nerves
% Cav1.2 cx	++	+	+	+	+	N/A
% VIAAT cx	++	++	++	++	+	N/A
% Cav1.3 cx	+	+	++	+	-	N/A
% VIAAT cx	+	+	++	++	-	N/A
% Cav1.4 cx	N/A	N/A	N/A	N/A	+	N/A
% VIAAT cx	N/A	N/A	N/A	N/A	+	N/A
% Cav2.1 cx	+	+	-	+	++	N/A
% VIAAT cx	++	+++	+	++	+++	N/A
% Cav2.2 cx	++	+	+	+	++	N/A
% VIAAT cx	++	++	++	++	++	N/A
% Cav2.3 cx	N/A	+	+	+	N/A	N/A
% VIAAT cx	N/A	+	+	+	N/A	N/A
% Cav3.1 cx	-	+	+	+	+	N/A
% VIAAT cx	-	+	+	+	+	N/A
% Cav3.2 cx	+	+	+	+	+	N/A
% VIAAT cx	+	+	+	+	++	N/A

For each coexpression (cx) experiment, the relative percentage of cells expressing both the VGCC subunit and xVIAAT (yellow FISH signal in Figs. 4) out of the total number of cells expressing the VGCC subunit (green and yellow FISH signal in Figs. 4) was determined. Additionally, the relative percentage of cells expressing both the VGCC subunit and xVIAAT (yellow FISH signal) out of the total number of cells expressing xVIAAT (red and yellow FISH signal) was determined. A single plus sign (+) indicates that <25% of the cells expressing the marker displayed coexpression. Two plus signs (++) indicates that 25–75% of the cells expressing the marker displayed coexpression, and three plus signs (+++) indicates >75% coexpression. Experiments without coexpression are marked with a minus sign (−).