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. 2014 Jun 3;9(6):e98657. doi: 10.1371/journal.pone.0098657

Figure 1. Effect of WT and 112-syn on heat induced aggregation of aldolase (ALD).

Figure 1

(A) In vitro protein aggregation assay employing aldolase (0.1 mg/mL in PBS) was performed at 65°C in the absence or presence of WT and 112-syn at different ratios (1∶2 and 1∶4) and the light scattering was monitored at 360 nm for a period of 10 min. (B) The net change in the absorbance of reaction mixtures of (A) before and after 10 min of incubation at 65°C. Data are the mean ± SD of three separate experiments. * indicates p<0.01 as compared to aldolase (decrease); #p<0.01 as compared to aldolase (increase) and $ p<0.01 as compared to the respective controls (before heating).