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. 2014 Jun 3;9(6):e98657. doi: 10.1371/journal.pone.0098657

Figure 4. Time dependent fibrillation of WT and 112-syn.

Figure 4

Time dependent fibrillation of WT-syn (A) and 112-syn (B) was monitored by the enhancement of ThT fluorescence. WT and 112-syn (0.5 mg/mL) at 37°C were incubated in 20 mM Tris buffer, pH 7.5 for indicated time intervals as described in the “Methods” section and the ThT fluorescence emission spectrum was recorded. (C) Fold change in the ThT fluorescence of WT and 112-syn incubated for different time intervals. Data presented are the mean ± SD of three separate experiments. (D) Aliquots from reaction mixtures incubated at 37°C for 24 h were adsorbed onto carbon coated grids and then stained with 1% uranyl acetate for 1–2 min. Negatively stained TEM images of WT and 112-syn are shown. Scale bars, 500 nm and data is a representative of three different fields of view. *p<0.01 as compared to 0 h readings for 112-syn.