Figure 2. Effect of radiation on endothelial cell migration as assessed by the Boyden chamber migration assay.

Representative images are shown of migrating HMEC-1 cells exposed to conditioned medium from cultures of A. unirradiated or heavy ion-irradiated, or B. unirradiated or X ray-irradiated glioma cells. C. Quantification of the number of migratory endothelial cells is expressed as means ± standard error of the mean from three independent experiments, in which treatments were performed in triplicate. *P<0.05, ^***P<0.001 vs. controls (0 Gy).