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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Hepatology. 2014 May 1;59(6):2251–2262. doi: 10.1002/hep.27026

Fig. 4.

Fig. 4

DNMT1 sh-RNA knockdown in Huh7 cells increases self-renewal potential. (A) Immunoblotting of DNMT1 and DNMT3a. HeLA nuclear extract served as a positive control for DNMT1 expression. β-actin used as a loading control. (B) Sphere frequency at G1-G5 generations. The data are means ± SEM (n=3, ***P<0.001 by Poisson GLM with multiple comparison test). (C) Limiting dilution analysis. The frequency of tumor initiating cells (TIF) and confidence interval (CI95%) were calculated based on the number of resulting tumors/injection site at 10 weeks. (D) Self-renewal of WRL68 cells expressing low basal levels of DNMT1 protein. The data are means ± SEM (n=3, Poisson GLM with multiple comparison test). (E) Immunoblotting of DNMT1. β-actin used as a loading control.