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. Author manuscript; available in PMC: 2015 Jun 15.

Published in final edited form as: Arch Biochem Biophys. 2013 Dec 26;0:29–39. doi: 10.1016/j.abb.2013.12.011

TABLE 2.

In Vitro motility assays:

Maximum velocities
Myosin	V_actin (μm/s)	WT Comparison	D166V Comparison
WT	0.69 ± 0.02	-----	No difference
S15A	0.67 ± 0.02	No difference	No difference
S15D	0.67 ± 0.02	No difference	No difference
D166V	0.69 ± 0.02	No difference	-----
S15A-D166V	0.71 ± 0.02	No difference	No difference
S15D-D166V	0.75 ± 0.03	No difference	No difference

Myosin driving forces
Myosin	Force (μN)	WT Comparison	D166V Comparison
WT	4.30 ± 0.48	-----	↑ (P < 0.0005)
S15A	3.22 ± 0.30	No difference	↑ (P < 0.005)
S15D	3.11 ± 0.33	No difference	↑ (P < 0.05)
D166V	2.04 ± 0.20	↓ (P < 0.0005)	-----
S15A-D166V	1.81 ± 0.18	↓ (P < 0.0001)	No difference
S15D-D166V	3.13 ± 0.36	No difference	↑ (P < 0.005)