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. 2014 Jun 3;9(6):e98658. doi: 10.1371/journal.pone.0098658

Figure 3. CMZ prevented the decrease of protein levels of PPAR-α, p300 and Sirt-1, and inhibited the increased acetylation of PGC-1α induced by ethanol.

Figure 3

Total protein samples were prepared using RIPA buffer, and protein levels of PPAR-α, RXR-α, p300, PGC-1α, and Sirt-1 were detected by western blot. The acetylation of PGC-1α was determined by immunoprecipitation analysis. The mRNA levels of PPAR-α, RXR-α, and PGC-1α were measured using qPCR. (a) Protein levels of PPAR-α, RXR-α, p300, and PGC-1α; (b) Protein levels of Sirt-1; (c) Acetylation of PGC-1α; (d) mRNA levels of PPAR-α, RXR-α, and PGC-1α. Data were presented as mean ± SD from at least 3 independent experiments, and expressed as the percentage of the control. *P<0.05, **P<0.01, compared with control group; # P<0.05, ## P<0.01, compared with ethanol group.