Table 1. Cytoskeletal proteins and proteins known to interact with CCM complex.
| Gene/sequence ID Protein description | Condition(s) with highest expression | Function | Subunits |
| EF1A1/P10126 b Elongation factor 1-alpha 1 | Cell line 1, cell line 2 | Enhances the binding of amino-acyl tRNA to the ribosome A-site, a GTP-dependent process | Binds to CCM protein complex |
| EF1G/Q9D8N0 Elongation factor 1-gamma | Cell line 1, cell line 2, cell line 3 | Probably plays a role in anchoring the complex to other cellular components | EF-1 is composed of four subunits: alpha, beta, delta, and gamma |
| TCPB/P80314 a , b T-complex protein 1 subunit beta | Cell line 1, cell line 2, cell line 3 | Serves as a molecular chaperone by assisting the folding of proteins upon ATP hydrolysis. Known to help in folding of actin and tubulin, in vitro | Exists as heterooligomeric complex (850 to 900 kDa) which forms two stacked rings |
| TCPG/P80318 a , b T-complex protein 1 subunit gamma | Cell line 1, cell line 2, cell line 3 | Serves as a molecular chaperone by assisting the folding of proteins upon ATP hydrolysis. Known to help in folding of actin and tubulin, in vitro. Plays a part in the assembly of the von Hippel–Lindau ubiquitination complex | Exists as heterooligomeric complex (850 to 900 kDa) which forms two stacked rings |
| TAGL/P37804 a Transgelin | Cell line 1, cell line 2, cell line 4 | Functions in actin cross-linking/gelling protein | |
| ENOA/P17182 b Enolase | Cell line 1, cell line 2, cell line 3 | Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. By similarity. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production | Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding by similarity. In vitro, interacts with several glycolytic enzymes including PKM2, PGM, CKM and aldolase. Also binds troponin, in vitro. |
| GBLP/P68040 b Guanine nucleotide-binding protein subunit beta-2-like 1 | Cell line 1, cell line 2, cell line 3 | Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. | Component of the small (40S) ribosomal subunit. Exists as a monomer and also forms oligomers. Binds SLC9A3R1. Forms a ternary complex with TRIM63 and PRKCE. Interacts with HABP4, KRT1 and OTUB1. Interacts with SRC (via SH2 domain); the interaction is enhanced by tyrosine phosphorylation of GNB2L1/RACK1. Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and PRKCA. Interacts with AR. Interacts with IGF1R but not with INSR. Interacts with ADAM12. Interacts with CLEC1B (via N-terminal region) and with HIF1A; the interaction promotes their degradation. Interacts with RHOA; this enhances RHOA activation and promotes cell migration. Interacts with CHRM2; the interaction regulates CHRM2 internalization. Interacts with TRPM6 (via kinase domain). Interacts with PTK2; required for PTK2 phosphorylation and dephosphorylation. Interacts with FLT1. |
| MYH9/Q8VDD5 a Myosin-9 | Cell line 1, cell line 3 | Plays a role in cytokinesis, cell shape, and specialized functions such as secretion and capping | Exists as a hexamer consisting of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2) |
| TBA1A/P68369 a , b Tubulin alpha-1A chain | Cell line 1, cell line 2, cell line 3 | Serves as the major constituent of microtubules. It binds two moles of GTP, one located on an exchangeable site on the beta chain and the other at a non-exchangeable site on the alpha chain | Exists as a dimer of alpha and beta chains |
| TBA1B/P05213 a , b Tubulin alpha-1B chain | Cell line 1, cell line 2, cell line 3 | Serves as the major constituent of microtubules. It binds two moles of GTP, one located on an exchangeable site on the beta chain and the other at a non-exchangeable site on the alpha chain | Exists as a dimer of alpha and beta chains |
| TBB5/P99024 a , b Tubulin beta-5 chain | Cell line 1, cell line 2, cell line 3 | Serves as the major constituent of microtubules. It binds two moles of GTP, one located on an exchangeable site on the beta chain and the other at a non-exchangeable site on the alpha chain | Exists as a dimer of alpha and beta chains |
aCytoskeletal proteins.
bProteins associated with CCM protein complex (Hilder et al. 2007).