Fig. 3.
The effects of sucrose on the rate of breaking of actin filaments during a motility assay. The time it takes a given actin filament to break was measured during a motility assay. Approximately 100 measurements under each condition were plotted in a histogram and fit to a single exponential to determine filament breaking rates both in the absence (□, solid line) and presence (○, dashed line) of 880 mM sucrose. Sucrose decreased the rate of breaking from 0.052 to 0.017 s−1.