Fig. 5.

The effects of sucrose on the diffusion coefficient of actin fragments in SiMBA. (A) Mean squared displacements (MSD) from over 300 actin fragment trajectories were measured at different time windows for 0 (□), 440 (○), and 880 (△) mM sucrose. (B) Diffusion coefficients were determined at each sucrose concentration by fitting the MSD data to 2Dt^α and plotted. D = diffusion coefficient, t = time, α = alpha coefficient. Results are summarized in Table 1.