Table 1.

Summary of kinetic parameters measured from in vitro motility, actin-activated S1 ATPase, single molecule binding, and stopped-flow spectroscopy assays.

Kinetic parameters with and without sucrose
Sucrose:	0 mM	880 mM
V, μm·s−1	2.05 ± 0.3 (3)	0.43 ± 0.2 (3)
D actin, μm2·s−1	0.72 ± 0.02	0.54 ± 0.02
kcat*, min−1	75 ± 13 (2)	15 ± 1 (2)
Km *, μM	33 ± 2 (2)	9 ± 1 (2)
ρ·katt(−ATP) †, s−1	0.72 ± 0.02	0.21 ± 0.02
ρ·katt(+ATP) †, s−1	0.97 ± 0.08	0.14 ± 0.01
katt(−ATP) ‡, μM−1·s−1	2.41 ± 0.08	0.48 ± 0.01
kdet(−ATP) ‡, s−1	0.13 ± 0.06 (2)	0.11 ± 0.03 (2)
kdet(−ATP) †, s−1	1.3 ± 0.2	1.0 ± 0.1
kT ‡, μM−1·s−1	2.1 ± 0.1	1.3 ± 0.1
KADP ‡, μM	217 ± 30	244 ± 33
Ea(att) ‡, kJ·mol−1	50 ± 2	68 ± 5
Ea(det) ‡, kJ·mol−1	24 ± 3	28 ± 3

^*Steady-state ATPase assays performed at 700 mM sucrose.

^†SiMBA

^‡Stopped-Flow

N values are in parenthesis.

Errors with (N) are ± SD. All other errors are SEM to a fit.