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. 2014 Jun 3;9(6):e98524. doi: 10.1371/journal.pone.0098524

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© 2014 Soliman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Subcellular localization of HvBT1 was visualized by Zeiss confocal Laser scanning microscope. A: transient expression of HvBT1::YFP in living protoplasts; chlorophyll autoflorescence (red color), YFP fluorescence (yellow color) and merged image (orange color). B: immunolocalization of HvBT1::YFP was detected using anti-YFP antibody and visualized by the fluorescence of FITC-conjugated antibody. Images represent FITC fluorescence (blue color), bright field (grey image) and the merged image that show the localization of HvBT1::YFP on the chloroplasts membranes.