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. 2014 Jun 3;9(6):e98524. doi: 10.1371/journal.pone.0098524

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© 2014 Soliman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Escherichia coli C43 and rosetta2 strains harboring the original (org) or the optimized (opc) ORF of HvBT1 were grown in 2xYT liquid media supplied with IPTG (0.5 mM final concentration). His-tagged HvBT1 membrane protein was purified and subjected to 12% SDS-PAGE. Lane 1 and 2 represent Rosetta 2 harboring opc and org ORF of HvBT1, respectively. Lane 3 and 4 represent C43 harboring opc and org ORF of HvBT1, respectively. Black arrows point to a band size of 45 KDa. Protein standard molecular weight is shown.