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. Author manuscript; available in PMC: 2015 Mar 28.
Published in final edited form as: J Nat Prod. 2013 Dec 3;77(3):527–535. doi: 10.1021/np400800h

Figure 7.

Figure 7

Effect of 1 and LY294002 on LPS-induced protein expression of Oct-2 and iNOS in RAW 264.7 cells. Time-course of Oct-2 protein expression after LPS treatment with or without 1-pretreatment was examined. RAW 264.7 cells incubated with 1 μg/mL LPS for 1, 2, 4, 6, 8, and 20 h were lysed to determine protein expression levels of Oct-2 using Western blot analyses (A). After normalization to the internal standard GAPDH, % expression of the 1 (25 μM) pretreatment group relative to Oct-2 expression in LPS-treated control (100%) is presented (n = 3) (B). The inhibition of LPS-induced iNOS protein expression by 1 and LY294002 has been examined after 20 h treatment with LPS (C). An asterisk (*) indicates a significant difference between LPS-treated control and 1-pretreated/LPS-treated control with p values less than 0.05.