FIG. 4.

Influence of the growth phase and different carbon sources on polyP accumulation by cells of Pseudomonas sp. strain B4 and B. fungorum LB400. (A) Cells of Pseudomonas sp. strain B4 (open bars) or B. fungorum LB400 (solid bars) were grown in LB medium or in M9 medium supplemented with glucose or biphenyl as the sole carbon source. Aliquots of the cells were analyzed to determine the polyP contents during the exponential (E) or stationary (S) phase of growth. The error bars indicate the standard deviations based on three different experimental values. (B) Polyacrylamide gel electrophoresis of polyP purified from Pseudomonas sp. strain B4 cells grown with biphenyl (lane 4, arrow). Lanes 1 to 3 contained polyP standards (lane 1, 45 residues; lane 2, 75 residues; lane 3, 750 residues).