TABLE 1.
Growth with biphenyl and CBs greatly enhances generation of ROSa
| Carbon source | Growth (FU/mg)c |
|---|---|
| Glucose (1%) | 16,837 |
| Biphenyl (2 mM) | 174,075 |
| 4-Chlorobiphenyl (2 mM) | 606,597 |
| 2-Chlorobiphenyl (2 mM) | 319,027 |
| Glucose (1%) + H2O2 (50 mM)b | 1,384,377 |
a
DCFH-DA (5 μM) was added to Pseudomonas sp. strain B4 cells growing exponentially with different carbon sources, and the preparations were incubated for 1 h in the dark.
b
As a positive control, cells grown with glucose were incubated for the same time in the presence of 50 mM hydrogen peroxide.
c
Fluorescence was measured as described in Materials and Methods and was expressed in fluorescence units (FU).