Figure 4. Cellular copper of KO cell line.

(A) Total cellular Cu was determined after 24 h or after chase (48 h). Cells were subjected to AAS analysis. Data are recorded for viable 10⁶ cells. (B) Cu concentrations of cell lysates from KO (black) and HepG2 cells (open) are shown. Subcellular fractions were derived by differential centrifugation after 48 h Cu exposure. Data is represented as mean±SE of three independent experiments. Asterisks indicate significance (p<0.05).