FIG. 3.
Phenotypic and genotypic analysis of a naturally occurring E. carotovora subsp. carotovora strain deficient in TTSS. (A) HR assay with tobacco (N. tabacum cv. Xanthi). The bacteria were infiltrated into tobacco leaves at a level of 2× 108 CFU/ml. Deionized sterile water was used as a negative control. (B) Southern analysis of genomic DNA isolated from representative E. carotovora subsp. carotovora strains and digested with EcoRI. The probe used for the blot was PCR amplified by using primers hrpN2 and hrc-bamF, which generated a 6.0-kb product spanning eight hrp and hrc genes. Ecc, E. carotovora subsp. carotovora; SG, serogroup.