TABLE 1.
Bacterial strains and plasmids used in this study
| Strain, serogroups, or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| E. coli DH5α | supE44 ΔlacU169 (Δ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Clontech |
| E. carotovora | ||
| WPP1 to WPP27 | Wild-type strains isolated from diseased potato tubers and stems from fields in Wisconsin during the summer of 2001 | This study |
| Serogroups 2 to 17, 19, 21, 23, 24, 26 to 40 | Strains isolated in the United States, Canada, and the Netherlands representing 35 common serogroupsa | 10 |
| WPP49 | Cmr; ΔrrnA::cat derivative of WPP14 | This study |
| WPP51 | Cmr Spr/Smr; ΔrrnA::cat ΔrrnB::aadA derivative of WPP49 | This study |
| WPP53 | Cmr Spr/Smr; ΔrrnA::cat ΔrrnC::aadA derivative of WPP49 | This study |
| WPP54 | Cmr Spr/Smr; ΔrrnA::cat ΔrrnD::aadA derivative of WPP49 | This study |
| WPP55 | Cmr Spr/Smr; ΔrrnA::cat ΔrrnE::aadA derivative of WPP49 | This study |
| WPP57 | Cmr Kmr; ΔrrnA::cat ΔrrnF::kan derivative of WPP49 | This study |
| Plasmids | ||
| pGEMT-Easy | AmprlacZ′ cloning vector | Promega |
| pHP45ΩSp/Sm | Ampr Spr/Smr broad-host-range cloning vector | 12 |
| pKD3 | Kmr template plasmid carrying kan cassette | 8 |
| pKD4 | Cmr template plasmid carrying cat cassette | 8 |
| pTΔ23S | Ampr pGEMT-Easy carrying 2.1-kb I-CeuI-restricted 23S ribosomal DNA of WPP14 | This study |
| pTΔ23SKm | Ampr Kmr pTΔ23S derivative harboring 1.6-kb kan of pKD4 cloned into BamHI site | This study |
| pTΔ23SCm | Ampr Cmr pTΔ23S derivative harboring 1.1-kb cat gene of pKD3 cloned into BamHI site | This study |
| pTΔ23SΩSp | Ampr Spr/Smr pTΔ23S derivative harboring 2-kb ΩaadA gene of pHP45ΩSp cloned into BamHI site | This study |
a
For strain designations see reference 10.