TABLE 2.

Primers used in this study

Primer	Sequence (5′→3′)	Targeted region	Restriction enzyme site(s)
rrnSac-A	GAG CTC AGG GTG ACT GCG TAC C	rrn	SacI
rrnBSce-B	ATT ACC CTG TTA TCC CTA GGA TCC TGG AAG CGT AGC A	rrn	BamHI, I-SceI
rrnBSce-C	GGA TCC TAG GGA TAA CAG GGT AAT GAT GGC CAG GCT G	rrn	BamHI, I-SceI
rrnERI-D	GGT TAA GCC TCT CGG GGA ATT CGT AC	rrn	EcoRI
G1	GAA GTC GTA ACA AGG	ITS region
L2	CAA GGC ATC CAC CGT	ITS region
D34BP-F	CTG CAG GAT CCG TGT AGG CTG GAG CTG CTT C	cat or kan pKD3/4	BamHI, PstI
D34BP-R	CTG CAG GAT CCC ATA TGA ATA TCC TCC TTA	cat or kan pKD3/4	BamHI, PstI
acnA1	GCC TCG CCG CCG CTG GTG GT	acnA
acnA2	CCG CGC ATC ATC ACT TCA TG	acnA
hemGfor	GGT CAT ACG CAT AAT GAA CTG AAT C	hemG
hemGrev	GCS AAT AMS CTS AAA GGR ACG	hemG
hrpN2	GTG TTG CCG GTA TCA CCC	hrp and hrc
hrcC-bamF	ACA AGC GCA AAA GCT GGG G	hrp and hrc
mdh2	GCG CGT AAG CCG GGT ATG GA	mdh
mdh4	CGC GGC AGC CTG GCC CAT AG	mdh
mobBforwd	TTA CTK AAG CAS STC ATC CC	mobB
mobBrev	CCY TCA ACC AGM AYC ARA TC	mobB
murIfor	AGG GCG AGA ATA CTA TCT CAC	murI
murIrev	CMA CGA CRG GGA AAK TAA AAC	murI
outH-Fwd	GCT GCT GGA AAT CAT GCT GG	outH
outH-Rev	GTC TCC GGC GAC GTG TGC AC	outH
pelB1	CAG CAC AAA CAG CAC CAG CG	pelB
pelB2	GGG CCA CCG TTG TTG GTG CA	pelB
purH-f1	GAG TMM ACR CGG CTC ATY TG	purH
purH-r1	CCC RAC BAT GGT BCG CTC	purH
rsmA-F1	GGA TCC GGC AAG CAG GAT AG	rsmA
rsmA-R1	TGC GTC CCG CGA ACA CGA G	rsmA
gmhB-f2	GAT ATG GCB GCT TCT TAT ATG GTG	gmhB
gmhB-r1	CCA TAT AAG AAG CVG CCA TAT C	gmhB
yieP-f1	ACC TGG TGG ATG ACR GAA G	yieP
yieP-r1	ACC GAA TGA AAT AGC GWS GC	yieP