FIG. 4.

RFLP patterns obtained from 16S rRNA gene and 16S rRNA analyses of the domain Archaea (a and b) and the genus Pseudomonas (c and d) for the reference and test soils. Bands described in the text are indicated by arrowheads. The bands which indicate that there are significant differences between reference and test soils are indicated by solid arrowheads. For clear identification, the fragment sizes (in base pairs) of bands are indicated. The numbers at the bottom indicate sampling days ([d]). The data for only one of three replicates are presented. A 1-kb marker (lane M) was used as a DNA size standard.