FIG. 1.

Detection limits of tunable surface microparticles and phosphate-based extraction-hybridization buffers for G. chapellei intact rRNA in both test tube and automated hybridization procedures. HS buffer, HS phosphate hybridization buffer; LS buffer, LS phosphate hybridization buffer. Test tube hybridizations were carried out for 15 min at room temperature. Total hybridization time for the automated routine was 180 s; each bolus of target rRNA was in contact with the tunable surface particles for only 30 s. Error bars indicate standard deviations.