TABLE 1.
Probea | Abbreviation | Sequence (5′-3′)b | Perfect match | GenBank accession no. | Bead region |
---|---|---|---|---|---|
Capture | |||||
S-G-Gbc-0825-a-A-14 | Gbc825 | TACCCGCRACACCT | Geobacter | L100-L104-01 | |
S-S-Gbc.chap-0432-a-A-15 | Gbc446 | TTAGCCACAATACAC | Geobacter chapellei | U41561 | L100-L125-01 |
S-S-Gbc.sulf-0382-a-A-15 | Gbs | TTAGCTCTCAATCAT | Geobacter sulfurreducens | U13928 | L100-L119-01 |
S-S-Gbc.met-0454-a-A-15 | Gbm | TTAACCCTCAATCAC | Geobacter metallireducens | L07834 | L100-L115-01 |
S-S-Gbc.brem-0445-a-A-15 | Gbb | ATTAGCCAGCCCCAT | Geobacter bremensis | U96917 | L100-L117-01 |
S-S-Gbc.pel-0445-a-A-15 | Gbp | ATTAACCGCACACAT | Geobacter pelophilus | U96918 | L100-L140-01 |
S-S-Dsv.des-0460-a-A-15 | Dvd | TTAGCACAACGTAGT | Desulfovibrio desulfuricans | M34113 | L100-L155-01 |
S-S-Dsv.gab-0409-a-A-15 | Dvgb | TATTCGCATCCTCGG | Desulfovibrio gabonensis | U31080 | L100-L147-01 |
S-S-Dsv.halo-0455-a-A-15 | Dvh | TTCGACTCTAATGGT | Desulfovibrio halophilus | U48243 | L100-L107-01 |
S-S-Dsv.grac-0414-a-A-22 | Dvg | TATTCGACCTCAAGG | Desulfovibrio gracilis | U53464 | L100-L130-01 |
Fluorescent beacon | TTGTGGTGGTGTGGT-Alexa-532 | L100-L128-01 | |||
Chaperone | |||||
S-S-Gbc.chap-0401-a-A-15 | GAGCTTTACGACCCG | G. chapellei, G. metallireducens, G. bremensis | |||
S-S-Dsv.des-0429-a-A-15 | GAGGTTTACGATCCG | D. desulfuricans |
Oligonucleotide probes are named according to the guidelines of Alm et al. (1).
All capture probes were synthesized with 5′-biotin and two C18 spacers. The fluorescent beacon probe was synthesized with 5′-biotin and a 3′-C7-amino modifier.