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. 2004 May;70(5):2621–2631. doi: 10.1128/AEM.70.5.2621-2631.2004

TABLE 1.

Oligonucleotide probes and bead regions used in this study

Probea Abbreviation Sequence (5′-3′)b Perfect match GenBank accession no. Bead region
Capture
    S-G-Gbc-0825-a-A-14 Gbc825 TACCCGCRACACCT Geobacter L100-L104-01
    S-S-Gbc.chap-0432-a-A-15 Gbc446 TTAGCCACAATACAC Geobacter chapellei U41561 L100-L125-01
    S-S-Gbc.sulf-0382-a-A-15 Gbs TTAGCTCTCAATCAT Geobacter sulfurreducens U13928 L100-L119-01
    S-S-Gbc.met-0454-a-A-15 Gbm TTAACCCTCAATCAC Geobacter metallireducens L07834 L100-L115-01
    S-S-Gbc.brem-0445-a-A-15 Gbb ATTAGCCAGCCCCAT Geobacter bremensis U96917 L100-L117-01
    S-S-Gbc.pel-0445-a-A-15 Gbp ATTAACCGCACACAT Geobacter pelophilus U96918 L100-L140-01
    S-S-Dsv.des-0460-a-A-15 Dvd TTAGCACAACGTAGT Desulfovibrio desulfuricans M34113 L100-L155-01
    S-S-Dsv.gab-0409-a-A-15 Dvgb TATTCGCATCCTCGG Desulfovibrio gabonensis U31080 L100-L147-01
    S-S-Dsv.halo-0455-a-A-15 Dvh TTCGACTCTAATGGT Desulfovibrio halophilus U48243 L100-L107-01
    S-S-Dsv.grac-0414-a-A-22 Dvg TATTCGACCTCAAGG Desulfovibrio gracilis U53464 L100-L130-01
Fluorescent beacon TTGTGGTGGTGTGGT-Alexa-532 L100-L128-01
Chaperone
    S-S-Gbc.chap-0401-a-A-15 GAGCTTTACGACCCG G. chapellei, G. metallireducens, G. bremensis
    S-S-Dsv.des-0429-a-A-15 GAGGTTTACGATCCG D. desulfuricans
a

Oligonucleotide probes are named according to the guidelines of Alm et al. (1).

b

All capture probes were synthesized with 5′-biotin and two C18 spacers. The fluorescent beacon probe was synthesized with 5′-biotin and a 3′-C7-amino modifier.