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Primer Design Rule Parameters
| Design Parameters | Values Used |
|---|---|
| allowable 3' ends | S[SW,WS][SW,WS,SS] |
| allowable 5' ends | WW, [WS,SW]SW |
| MINTEMP | 48 |
| MAXTEMP | 70 |
| MAXTEMPRANGE | 8 |
| MINCG% | 40 |
| MAXCG% | 60 |
| MINPRIMERLENGTH | 17 |
| MAXPRIMERLENGTH | 24 |
| MAXDEGENERACY | 64 |
| MAXSELFDIMER | 6 |
| MAXPRIMERDIMER | 6 |
| MAXRUNLENGTH (bp) | 4 |
| MINAMPLICONLENGTH | 150 |
| MAXAMPLICONLENGTH | 600 |
