Table 4.

Select Designed Primers

regionID	5'→3' sequence	deg	sDimer	TmRange	CG%Range	nHits	posEcoli
FV3	ACWCCTRCGGGWGGCWG	16	4	51.88→54.29	0.65→0.70	8867	345
FV3	ACWCCTRCGGGWGGCWGCAG	16	6	57.93→59.98	0.65→0.70	8854	345
FV4	AGCAGCCGCGGTAANACG	4	6	52.60→54.88	0.61→0.67	8305	528
FV6	WACSCGMRGAACCTTACC	16	6	48.04→52.60	0.50→0.61	8045	975
FV7	ATGGYYGTCGTCARCTCG	8	4	48.04→54.88	0.50→0.67	8821	1062
FV7	AGTCCNRYAACGAGCGCAACC	16	4	54.36→60.21	0.52→0.67	8788	1103
FV8	AGGAAGGHGDGGAYGASGTC	36	6	53.83→59.98	0.55→0.70	8810	1185
RV3	TTACCGCGGCTGCTGGCAC	1	6	57.56→57.56	0.68→0.68	8926	505
RV3	TWTYACCGCGGCTGCTGG	4	6	52.60→54.88	0.61→0.67	8543	508
RV4	ACCAGGGTATCTAAKCCTG	2	4	48.93→51.09	0.47→0.53	8292	773
RV6	TYACRRCACGAGCTGWCG	16	5	48.04→54.88	0.50→0.67	9026	1052
RV7	ACGTCRTCCHCWCCTTCC	12	4	50.32→54.88	0.56→0.67	8114	1166
RV8	TGWGTACAAGRYCCGRGAACG	16	6	52.40→58.26	0.48→0.62	8411	1367