Figure 4.

Loss of Pot1a results in chronic DNA damage. The inactivation of Pot1a in neural progenitors leads to an accumulation of DNA damage as shown by γH2AX immunostaining. DNA damage is found in different brain regions, including the developing cerebellum and cortex at postnatal day 7 and persists in older animals at 1 month. Arrows indicate representative γH2AX foci. EGL is of the cerebellum; WM is the cerebellar WM. DG, Dentate gyrus; CTX, cerebral cortex; CA1, cornu ammons region 1 of the hippocampus. Sections shown are from WT, Pota1a^Nes-cre, Pota1a^Nes-cre;Atm^−/−, and Pota1a^Nes-cre;p53^−/− mice.