rAAV/EnvA rabies virus labels individual cells in the cerebral cortex and other brain regions. rAAV2(YF4)-CBA-DIO-TVA (1.5 × 109 genome copies) and EnvA-SADΔG-GFP were injected into M1, S1,V1, LGN, and SC in two Cre drivers crossed with the Ai9 reporter: CCK-ires-Cre × Ai9 and SST-ires-Cre × Ai9. The doses of EnvA-SADΔG-GFP injected into each region were: 300 IU for M1, S1, V1, and SC in CCK-ires-Cre driver; 30 IU for M1, S1, V1, and SC in SST-ires-Cre driver; and 3 × 103 IU for the LGN in both drivers. Cre expression in each region is revealed by tdTomato labeling (red); GFP labeling of individual cells is shown in green. Insets, Coexpression of GFP (green) and tdTomato (red) in the somata. Scale bars, 100 μm.