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. 2014 Jun 4;34(23):7845–7861. doi: 10.1523/JNEUROSCI.2960-13.2014

Figure 4.

Figure 4.

The CRH-ires-Cre driver targets two amacrine cell types. A, B, CRH-1 amacrine cell. Flat-mount view of a CRH-1 amacrine cell is shown in A (inset shows the spiny dendrites), and side view with ChAT (blue) is shown in B. Scale bars: A, 50 μm; B, 10 μm. C–H, CRH-2 amacrine cell. A flat-mount retina with a single GFP-labeled CRH-2 wide-field amacrine cell is shown in C. Neurolucida tracing from C is shown in D, with the dendrites and soma drawn in green, the axon-like processes drawn in red, and the outline of the retina marked in white. The region around the soma (marked with the box in C) is enlarged in E, with soma and ON arbors labeled in green and OFF arbors pseudocolored in red. F, Side view with ChAT (blue) of a center region, including the soma, dendrites, and axon-like processes (boxed region in E). G, Side view of a proximal segment of the axon-like process near the soma (yellow arrow in E). H, Side view of a distal segment of the axon-like process (yellow arrow in C). I, J, CRH-1 (I) and CRH-2 (J) amacrine cells are both GABAergic. CRH amacrine cells (green) are colabeled with an antibody against GABA (red). scale bar 10 μm. Scale bars: C, D, 1 mm; E, in 50 μm; F–H, 10 μm.