Table 1.
Cre driver | Cell type | Titer of rAAVa (genome copies) | rAAV injection site | Titer of RVa (infectious units) | RV injection site | Timing of RV injection (post rAAV injection) |
---|---|---|---|---|---|---|
CRH | CRH-1 AC | 5 × 109 | Eye | 500–1000 | Eye | 5 weeks |
CRH | CRH-2 AC | |||||
VIP | VIP-1 AC | |||||
nNOS | nNOS-1 AC | |||||
nNOS | nNOS-2 AC | |||||
SST | SST-1 AC | |||||
CCK | SAC | |||||
CCK | A17 | |||||
CCK | CCK-1 RGC | 1 × 1010 | Eye | 106 | LGN or SC | 5 weeks |
CCK | CCK-2 RGC | |||||
CCK | CCK-3 RGC | |||||
CCK | M1 cells | 1.5 × 109 | M1 | 300 | M1 | 3 weeks |
CCK | S1 cells | S1 | S1 | |||
CCK | V1 cells | V1 | V1 | |||
CCK | SC cells | SC | SC | |||
SST | M1 cells | 1.5 × 109 | M1 | 30 | M1 | 3 weeks |
SST | S1 cells | S1 | S1 | |||
SST | V1 cells | V1 | V1 | |||
SST | SC cells | SC | SC | |||
CCK | LGN cells | 1.5 × 109 | LGN | 3000 | LGN | 3 weeks |
SST | LGN cells | 1.5 × 109 | LGN | 3000 | LGN | 3 weeks |
AC, Amacrine cell.
arAAV2 (YF4)-CBA-DIO- TVA.
bRabies virus EnvA-SADΔG-GFP.