Glutamatergic neurons in the substantia nigra send projections to the nRT and the PTh, but not the VPL. A, Schematic showing the conditional viral expression and the injection site in a vGluT2-Cre mouse. B–F, Successive sections showing the YFP labeling in the SN and thalamus, with the first panel corresponding to plate 13. Coordinates indicate steps, in micrometers, toward the midline. G–J, High-power images of the labeling observed in the SN and in different thalamic regions.