TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Descriptiona	Source or reference
Strains
E. coli
DH5α	F−recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ(argF-lacZYA) UI169 φ80lacZ ΔM15 λ−	GIBCO-BRL
HB101	F−hsdS20 (rB− mB−) supE44 recA1 ara14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-5 λ−	GIBCO-BRL
MC1061	F−araD139 Δ(araABC-leu)7679 galU galK Δ(lac)X74 rpsL thi	GIBCO-BRL
P. aureofaciens
30-84	Phz+ Rifr; wild type	W. W. Bockus
30-84Z	Phz− Rifr; phzB::lacZ genomic fusion	27
30-84I1/I2	Phz− Rifr; phzI::Kmr and csaI::Gmr genomic fusion	39
30-84Z/I	Phz− Rifr; phzB::lacZ, phzI::Kmr genomic fusions	38
30-84R	Phz− Rifr Kmr; phzR::Tn5lacZ genomic fusion	27
Plasmids
pLAFR3	IncP1 cos+rlx+ Tcr	31
pRK2013	IncP1 tra oriE1 Kmr	10
pDW7311uidA	pLAFR3 with phzI::uidA fusion	6
pLAF-2.7RVΔ3	pLAFR3 containing 2.2-kb genomic fragment containing phzI	37
pLSP10-21	pLAFR3 containing the 11.2-kb EcoRI fragment of pLSP259 with the phzI and phzR genes	27

^aRif^r, rifampicin resistant, Km^r, kanamycin resistant; Tc^r, tetracycline resistant; Ap^r, ampicillin resistant; Gm^r, gentamicin resistant.