TABLE 3.
Effect of conditioned medium on phzB expression in strain 30-84Z
| CM sourcea | β-Galactosidase activity (Miller units)b | Fold changec |
|---|---|---|
| 30-84I1/I2 (control)d | 193 ± 23 | |
| DH5α | 211 ± 18 | +1.1 |
| PU-5 | 44 ± 1 | −4.3 |
| PU-15 | 33 ± 5 | −5.8 |
| PU-43 | 34 ± 4 | −5.7 |
| PU-177 | 33 ± 2 | −5.8 |
| PU-295 | 21 ± 2 | −9.2 |
| PU-761 | 32 ± 5 | −6.0 |
| 30-84Ze | 466 ± 46 | +2.4 |
a
Strain used to prepare CM.
b
β-Galactosidase activity was measured after 24 h. Values shown are the average of results for three replicates ± standard errors.
c
Fold change indicates the level of β-galactosidase activity relative to that of the 30-84I1/I2 control.
d
Strain 30-84I1/I2 is a derivative of P. aureofaciens defective in phzI and csaI. It does not produce phenazines or AHL signals but does deplete the CM of nutrients.
e
Strain 30-84Z is a phzB::lacZ reporter that does not produce phenazines but does produce both P. aureofaciens AHL signals.