Fig. 3.

Stellate cells express molecular markers of GABA/glycine co-releasing interneurons. A: photomicrograph of the DCN in a GAD65-GFP mouse, showing that stellate cells express the GABA-synthesizing enzyme GAD65. Arrows point to stellate cells located at the ependymal edge of the DCN. B: photomicrograph of the DCN in a section from a GlyT2-GFP mouse. Arrows point to small labeled neurons at the ependymal edge of the brain stem, suggesting that stellate cells also express the plasma membrane transporter required for functional glycine release. The larger neurons in the DCN molecular layer are presumably cartwheel cells, whereas the intense background reflects the high density of glycinergic fibers throughout the DCN. Scale bar in A applies also to B.