TABLE 1.
Purification of the bacteriocin albusin B from R. albus 7
| Purification step | Total protein (mg)a | Total activity (AU)b | Sp act (AU/mg) | Yield (%) |
|---|---|---|---|---|
| Supernatant (10 liters) | 1,400 | 3,920 | 2.8 | 100 |
| 4× concn of 10-kDa filtrate | 11.1 | 343 | 31 | 8.8 |
| 40 to 60% (NH4)2SO4 precipitation | 1.6 | 205.8 | 128.6 | 5.3 |
| P6 column (6-kDa cut off) | 0.3 | 185.2 | 617.3 | 4.7 |
a
Protein concentration was analyzed by the MicroBCA protein assay kit (Pierce), using lysozyme as the standard.
b
AU, activity unit. Activity units are defined as the reciprocal of the highest dilution at which growth inhibition was detectable and are expressed on a per-milliliter basis.